Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: List of markers used for immunohistochemistry

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Concentration Assay, Plasmid Preparation

Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: Visualization of the treated area, the transition zone, and untreated area as direct comparison by SD-OCT and histology at post-operative day 4. Hematoxylin and eosin (HE) staining already reveals atrophy of the photoreceptor outer segments and the outer nuclear layer (ONL) limited to the scraping site. The inner nuclear layer (INL) is not affected. Immunohistochemistry staining of the rabbit retina shows proliferating cells detected via Ki67 staining (red) which colocalize in part with subretinal microglia/macrophages shown by isolectin B4 staining (yellow). A strong deposition of collagen IV (red) is found around the scraping site beneath the ONL. Pan cytokeratin staining (red) for RPE already appears at the scraping site. Cell nuclei are stained with DAPI (cyan). Scale bar for SD-OCT = 200 µm, for histological images = 100 µm

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Staining, Immunohistochemistry

Journal: Mucosal immunology

Article Title: FcαRI (CD89) is upregulated on subsets of mucosal and circulating NK cells and regulates IgA-class specific signaling and functions

doi: 10.1016/j.mucimm.2024.04.003

Figure Lengend Snippet: Representative polychromatic flow cytometry plots measuring CD89 expression on (A) human and (B) rhesus macaque (RM) samples from several tissues. (C) CD89 frequency of NK cells from human PBMC and CBMC. (D) CD89+ frequency of NK cells from RM PBMC, mesenteric lymph node (MLN), and colon samples. (E) Chip Cytometry imaging of jejunum from RM stained for pan-cytokeratin, CD89, NKG2A/C, CD68, and CD3. Red arrows indicate cells co-expressing CD159a and CD89. (F) mRNA transcripts for CD89 (FCAR), CD64 (FCGR1A), CD32 (FCGR2A), CD16 (FCGR3A), and FcμR (FCMR) receptors are found in peripheral human NK cells. (G) UMAP analyses showing distinct clustering of a subset of CD89+ NK cells. Mann-Whitney U-test used in (C). Kruskal-Wallis test used in (D) and (E). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: Primary antibodies included CD3-BUV395 (Clone SP34.2, BD Biosciences), CD68-FITC (Clone KP1, Bio-Rad), CD89-PE (Clone A59, BD Biosciences), CD159a-PE (Clone REA110, Miltenyi Biotec), CD45-BUV395 (Clone D058–1283, BD Biosciences), and pan-cytokeratin-FITC (Clone AE1-AE3, Novus Biologicals).

Techniques: Flow Cytometry, Expressing, Chip Cytometry, Imaging, Staining, MANN-WHITNEY